医療従事者の為の最新医療ニュースや様々な情報・ツールを提供する医療総合サイト

New Promega NanoBRET™ Technology Enables Real-Time Monitoring of Protein Interactions in Live Cells

2015年05月05日 PM10:00
このエントリーをはてなブックマークに追加


 

MADISON, Wis.

Promega Corporation announces the launch of NanoBRET™ Protein Interaction Assays, which use a new Bioluminescence Resonance Energy Transfer (BRET) technology that enables scientists to quantitatively measure protein:protein interactions in live cells.

Promega NanoBRET(TM) Protein Interaction Assays use new Bioluminescence Resonance Energy Transfer (B ...

Promega NanoBRET(TM) Protein Interaction Assays use new Bioluminescence Resonance Energy Transfer (BRET) technology that enables scientists to quantitatively measure protein:protein interactions in live cells. (Photo: Business Wire)

Traditional methods for studying interactions between proteins are commonly performed in vitro using only protein fragments and do not provide data in the context of the cellular environment. With NanoBRET Protein Interaction Assays, researchers can study both induction and inhibition of protein interactions in real time using full-length proteins expressed at physiologically relevant levels.

Conventional BRET measures the interaction of proteins using a bioluminescent donor fused to a protein of interest and a fluorescent acceptor fused to its binding partner; the donor does not excite the fluorophore using light, but transfers resonance energy through dipole-dipole coupling. The optimized NanoBRET Protein Interaction Assays use NanoLuc® Luciferase as the energy donor and HaloTag® protein as the energy acceptor. NanoBRET Technology has improved spectral overlap, increased signal, and lower background, providing researchers with a reproducible method for monitoring and screening protein interactions. In addition, the brighter light output from NanoLuc enables use of NanoBRET even at low expression levels, while still providing efficient energy transfer.

To learn more about NanoBRET™ Protein Interaction Assays visit: http://www.promega.com/NanoBRET-PPItechnology

Promega Corporation is a leader in providing innovative solutions and technical support to the life sciences industry. The company’s 3,000 products enable scientists worldwide to advance their knowledge in genomics, proteomics, cellular analysis, drug discovery and human identification. Founded in 1978, the company is headquartered in Madison, WI, USA with branches in 16 countries and over 50 global distributors. For more information about Promega, visit www.promega.com.

Photos/Multimedia Gallery Available: http://www.businesswire.com/multimedia/home/20150505005084/en/

CONTACT

Promega Corporation
Penny Patterson
Sr. Director,
Communications
Phone: (608) 274-4330
E-mail: penny.patterson@promega.com

Related Posts Plugin for WordPress, Blogger...

同じカテゴリーの記事 

  • 印尼生命科学与技术学院在NCRM NICHE 2021上赢得第16届Fujio Cup Quiz大赛;印度Rajalakshmi工程学院获得亚军
  • 辉凌将在ACG 2021上展示基于微生物群的在研活体生物治疗药物RBX2660用于治疗艰难梭菌反复感染和IBD患者的获奖真实临床转归分析
  • ShouTi Secures $100 Million Series B Financing to Accelerate Development of Life-Changing Medicines Fueled by Advanced Computational, Structure-Based Drug Design Platform
  • 硕迪B轮成功融资1亿美元,利用先进的计算和结构药物设计平台加速开发改变患者命运的药物
  • CANbridge Pharmaceuticals CAN103 Investigational New Drug Application Approved in China for Treatment of Gaucher Disease